Somatic-Expert

On-farm mastitis test solution SOMATIC-EXPERT

Rapid detection of mastitis in lactating cows

Classic mastitis test (blue)

Based on milk somatic cell count

Detection of ruminal acidosis

Ready to use

Easy to use

Cost-effectiveness (2 ml per test)

Highly sensitive (detection threshold of 150,000 cells per ml of milk)

Quick results (in 10 sec after preparing testing mix)

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Application:

On-farm mastitis test SOMATIC-EXPERT is ready-to-use solution intended for in vitro detection of subclinical mastitis in cattle.

Description of mastitis detection principle:

Detection of mastitis using the test solution is based on the California mastitis test, the "gold" standard of international veterinary practice. The high sensitivity of the test is determined simultaneos evaluation of two characteristics of the sample: 1) apparent viscosity and 2) intensity of the dye color. Upon mixing the test solution with milk, the destruction of somatic cells occurs. It leads to viscosity increase, which is correlated to the number of somatic cells in the sample. Beside this, the mastitis test solution contains pH indicator that changes its color depending on the number of somatic cells in the sample. The intense contrast violet color appears in mastitis milk, while normal milk has a uniform non-intense blue-violet color.

Test procedure:

Place the examination paddle with four cups so that the handle of the paddle always points towards cow's head or tail so that the udder lobes and cups of the paddle are correctly aligned.

Discard the first three streams of milk into separate container.

Take an equal amount of milk (for example 2 ml each) from all quarters of the udder into corresponding cups of the paddle. To ensure that you have the same amount of milk in all cups, tilt the paddle vertically and let excessive milk to flow out. Avoid mixing the milk from different cups of the paddle.

Using a dispenser, add test solution to each cup in amount equivalent to the volume of the milk sample (for example 2 ml). Avoid making foam.

Mix solution and milk by gently rotating paddle in horizontal plane, thus forming the mixture. After 10 seconds or more, check test results.

Grading and Interpretation:

Observe changes in viscosity and color intensity in the cups. To interpret the results, refer to the tables. As a rule, viscosity and intensity of purple color increase in proportion to the concentration of somatic cells in the sample.

Table 1

#	Description of visible reaction	Possible interpretation	Somatic cell concentration
1	Mixture remains liquid with no evidence of formation of a precipitate and has uniform color	Negative	<150 000
2	A thread-like precipitate forms, wich is easy to see tipping the paddle back and forth in horizontal plane. The increase in viscosity observed during mixing may disappear.	Trace	150 000 – 400 000
3	The mixture is viscous, but no gel forms. Mixture pours out freely despite increased viscosity.	Weak positive	400 000 – 800 000
4	A gel is formed. When the rotation of the plate is stopped, the gel collects in the central part of the cup. When pouring out, the viscous mass falls out of the cup, while some liquid may remain in the cup.	Distinct positive	800 000 – 5 000 000
5	A viscous gel is formed, the level of which is raised in the center of the cup. The gel falls out when poured , leaving no liquid in the cup.	Strong positive	>5 000 000

Table 2

Color	Possible interpretation	Acidity of milk
Uniform non-intense blue-violet color	Lack of inflammation	pH 6.6 (normal)
Intense contrasting purple coloration	Suppression of secretory activity, which may be a consequence of an inflammatory process or in the proximity of the dry period	pH 7.0 or over (alkaline milk)
Yellow color	A rare phenomenon that indicates dysbiosis and formation of excess lactic acid by mammary gland bacteria. The presence of colostrum can also result in yellow coloration	pH 5.2 (acid milk)

In case when inflammation has just begun and interpretation of the results is difficult, it is recommended to repeat the test before the next milking. Please please take into account that very first trickles of milk should be used for the test, which allows to observe more intensive reaction and to see differences between healthy and slightly inflamed udder lobes.

Safety information:

When working with the diagnostic solution, follow general safety and personal hygiene requirements.

Storage conditions:

Test solution is stored in a dark place at a temperature between 2 °C and 35 °C.

Shelf-life:

Shelf life is 24 months from the date of production.

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